{"id":872,"date":"2019-09-18T12:45:41","date_gmt":"2019-09-18T10:45:41","guid":{"rendered":"https:\/\/www.festivalvedy.sk\/wp2\/idealna-teplota-eliminovania-inkluznych-teliesok-s-maximalnym-ziskom-proteinu-aslov2\/"},"modified":"2019-09-18T12:45:41","modified_gmt":"2019-09-18T10:45:41","slug":"idealna-teplota-eliminovania-inkluznych-teliesok-s-maximalnym-ziskom-proteinu-aslov2","status":"publish","type":"post","link":"https:\/\/www.festivalvedy.sk\/wp2\/idealna-teplota-eliminovania-inkluznych-teliesok-s-maximalnym-ziskom-proteinu-aslov2\/","title":{"rendered":"Ide\u00e1lna teplota eliminovania inkl\u00faznych teliesok s maxim\u00e1lnym ziskom prote\u00ednu AsLOV2"},"content":{"rendered":"<p>Autor\/autori: S\u00e1ra M\u00e1ria Majern\u00edkov\u00e1  <\/p>\n<p>\u00davod<br \/>\nLOV dom\u00e9ny patria k svetlocitliv\u00fdm prote\u00ednom, ktor\u00e9 vplyvom modr\u00e9ho svetla vytv\u00e1raj\u00fa kovalentn\u00e9 v\u00e4zby s flav\u00edn-mononukleotidom, \u010do sp\u00f4sob\u00ed zmenu konform\u00e1cie prote\u00ednovej \u0161trukt\u00fary. Dan\u00fd proces je reverzibiln\u00fd v tme a preto m\u00e1 vysok\u00e9 potenci\u00e1lne vyu\u017eite v optogenetike. Av\u0161ak produkcia t\u00fdchto prote\u00ednov m\u00f4\u017ee sp\u00f4sobi\u0165 ich hromadenie, pr\u00edpadne nespr\u00e1vne zbalenie v cytoplazme (vznik inkl\u00faznych teliesok). Vplyv teploty na tento proces st\u00e1le nie je dostato\u010dne presk\u00faman\u00fd. Preto je cie\u013eom tejto pr\u00e1ce overi\u0165 hypot\u00e9zu, \u017ee r\u00f4zna teplota prostredia m\u00f4\u017ee rozdielne vpl\u00fdva\u0165 na tvorbu inkl\u00faznych teliesok po\u010das indukovanej expresie LOV2 dom\u00e9ny.<br \/>\nMet\u00f3dy<br \/>\nBunky XL-1 Blue s implantovan\u00fdmi plazmidmi (pQIqH103C) sme pestovali v TB m\u00e9diu. N\u00e1sledne sa k bakt\u00e9ri\u00e1m pridalo IPTG, ktor\u00e9 indukovalo expresiu prote\u00ednu AsLOV2. Proces sme sledovali pri vybran\u00fdch teplot\u00e1ch (19\u00b0C, 25\u00b0C, 31\u00b0C, 37\u00b0C). V\u0161etky vzorky sa scentrifugovali a n\u00e1sledne sa verifikovala pr\u00edtomnos\u0165 inkl\u00faznych teliesok v supernatantoch a peletoch pomocou SDS-PAGE.<br \/>\nV\u00fdsledky<br \/>\nPod\u013ea parametra optickej denzity sme vypo\u010d\u00edtali po\u010det z\u00edskan\u00fdch buniek pri sk\u00faman\u00fdch kultiva\u010dn\u00fdch teplot\u00e1ch nasledovne: 2,16; 2,56; 3,76; 4,48 (*10^9\/1ml). Najvy\u0161\u0161iu koncentr\u00e1ciu AsLOV2 a minim\u00e1lnu pr\u00edtomnos\u0165 inkl\u00faznych teliesok sme verifikovali pri 19\u00b0C a 25\u00b0C. Najefekt\u00edvnej\u0161ia bunkov\u00e1 produkcia prote\u00ednu s minim\u00e1lnym vznikom inkl\u00faznych teliesok bola pri 25\u00b0C.<br \/>\n Z\u00e1ver<br \/>\nT\u00e1to pr\u00e1ca potvrdila vplyv teploty na tvorbu inkl\u00faznych teliesok v cytoplazme buniek. Expresia prote\u00ednov prebieha pri v\u0161etk\u00fdch \u0161tudovan\u00fdch teplot\u00e1ch, av\u0161ak len pri 19\u00b0C a 25\u00b0C do\u0161lo k efekt\u00edvnej elimin\u00e1cii vzniku ne\u017eiad\u00facich inkl\u00faznych teliesok. Optimaliz\u00e1cia podmienok expresie AsLOV2 dom\u00e9ny, t.j. minimalizovanie tvorby inkl\u00faznych teliesok pri zachovan\u00ed dostato\u010dnej expresie prote\u00ednu, m\u00e1 v\u00fdznam pre z\u00e1kladn\u00fd aj aplikovan\u00fd v\u00fdskum.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Autor\/autori: S\u00e1ra M\u00e1ria Majern\u00edkov\u00e1 \u00davod LOV dom\u00e9ny patria k svetlocitliv\u00fdm prote\u00ednom, ktor\u00e9 vplyvom modr\u00e9ho svetla vytv\u00e1raj\u00fa kovalentn\u00e9 v\u00e4zby s flav\u00edn-mononukleotidom, \u010do sp\u00f4sob\u00ed zmenu konform\u00e1cie prote\u00ednovej \u0161trukt\u00fary. Dan\u00fd proces je reverzibiln\u00fd v tme a preto m\u00e1 vysok\u00e9 potenci\u00e1lne vyu\u017eite v optogenetike. Av\u0161ak produkcia t\u00fdchto prote\u00ednov m\u00f4\u017ee sp\u00f4sobi\u0165 ich hromadenie, pr\u00edpadne nespr\u00e1vne zbalenie v cytoplazme (vznik inkl\u00faznych [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"categories":[6,12],"tags":[8,13],"class_list":["post-872","post","type-post","status-publish","format-standard","hentry","category-6","category-biologia","tag-8","tag-biologia"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.2 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Ide\u00e1lna teplota eliminovania inkl\u00faznych teliesok s maxim\u00e1lnym ziskom prote\u00ednu AsLOV2 - Festival vedy a techniky AMAVET<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.festivalvedy.sk\/wp2\/idealna-teplota-eliminovania-inkluznych-teliesok-s-maximalnym-ziskom-proteinu-aslov2\/\" \/>\n<meta property=\"og:locale\" content=\"sk_SK\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Ide\u00e1lna teplota eliminovania inkl\u00faznych teliesok s maxim\u00e1lnym ziskom prote\u00ednu AsLOV2 - Festival vedy a techniky AMAVET\" \/>\n<meta property=\"og:description\" content=\"Autor\/autori: S\u00e1ra M\u00e1ria Majern\u00edkov\u00e1 \u00davod LOV dom\u00e9ny patria k svetlocitliv\u00fdm prote\u00ednom, ktor\u00e9 vplyvom modr\u00e9ho svetla vytv\u00e1raj\u00fa kovalentn\u00e9 v\u00e4zby s flav\u00edn-mononukleotidom, \u010do sp\u00f4sob\u00ed zmenu konform\u00e1cie prote\u00ednovej \u0161trukt\u00fary. Dan\u00fd proces je reverzibiln\u00fd v tme a preto m\u00e1 vysok\u00e9 potenci\u00e1lne vyu\u017eite v optogenetike. Av\u0161ak produkcia t\u00fdchto prote\u00ednov m\u00f4\u017ee sp\u00f4sobi\u0165 ich hromadenie, pr\u00edpadne nespr\u00e1vne zbalenie v cytoplazme (vznik inkl\u00faznych [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.festivalvedy.sk\/wp2\/idealna-teplota-eliminovania-inkluznych-teliesok-s-maximalnym-ziskom-proteinu-aslov2\/\" \/>\n<meta property=\"og:site_name\" content=\"Festival vedy a techniky AMAVET\" \/>\n<meta property=\"article:published_time\" content=\"2019-09-18T10:45:41+00:00\" \/>\n<meta name=\"author\" content=\"Redakcia\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Autor\" \/>\n\t<meta name=\"twitter:data1\" content=\"Redakcia\" \/>\n\t<meta name=\"twitter:label2\" content=\"Predpokladan\u00fd \u010das \u010d\u00edtania\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 min\u00faty\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/www.festivalvedy.sk\/wp2\/idealna-teplota-eliminovania-inkluznych-teliesok-s-maximalnym-ziskom-proteinu-aslov2\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/www.festivalvedy.sk\/wp2\/idealna-teplota-eliminovania-inkluznych-teliesok-s-maximalnym-ziskom-proteinu-aslov2\/\"},\"author\":{\"name\":\"Redakcia\",\"@id\":\"https:\/\/www.festivalvedy.sk\/wp2\/#\/schema\/person\/f6c2e17170a626260fe38db97ed0a1f5\"},\"headline\":\"Ide\u00e1lna teplota eliminovania inkl\u00faznych teliesok s maxim\u00e1lnym ziskom prote\u00ednu AsLOV2\",\"datePublished\":\"2019-09-18T10:45:41+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/www.festivalvedy.sk\/wp2\/idealna-teplota-eliminovania-inkluznych-teliesok-s-maximalnym-ziskom-proteinu-aslov2\/\"},\"wordCount\":349,\"commentCount\":0,\"publisher\":{\"@id\":\"https:\/\/www.festivalvedy.sk\/wp2\/#organization\"},\"keywords\":[\"2019\",\"Biol\u00f3gia\"],\"articleSection\":[\"Arch\u00edv 2018\",\"Biol\u00f3gia\"],\"inLanguage\":\"sk-SK\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.festivalvedy.sk\/wp2\/idealna-teplota-eliminovania-inkluznych-teliesok-s-maximalnym-ziskom-proteinu-aslov2\/\",\"url\":\"https:\/\/www.festivalvedy.sk\/wp2\/idealna-teplota-eliminovania-inkluznych-teliesok-s-maximalnym-ziskom-proteinu-aslov2\/\",\"name\":\"Ide\u00e1lna teplota eliminovania inkl\u00faznych teliesok s maxim\u00e1lnym ziskom prote\u00ednu AsLOV2 - Festival vedy a techniky AMAVET\",\"isPartOf\":{\"@id\":\"https:\/\/www.festivalvedy.sk\/wp2\/#website\"},\"datePublished\":\"2019-09-18T10:45:41+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/www.festivalvedy.sk\/wp2\/idealna-teplota-eliminovania-inkluznych-teliesok-s-maximalnym-ziskom-proteinu-aslov2\/#breadcrumb\"},\"inLanguage\":\"sk-SK\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.festivalvedy.sk\/wp2\/idealna-teplota-eliminovania-inkluznych-teliesok-s-maximalnym-ziskom-proteinu-aslov2\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.festivalvedy.sk\/wp2\/idealna-teplota-eliminovania-inkluznych-teliesok-s-maximalnym-ziskom-proteinu-aslov2\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/www.festivalvedy.sk\/wp2\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Ide\u00e1lna teplota eliminovania inkl\u00faznych teliesok s maxim\u00e1lnym ziskom prote\u00ednu AsLOV2\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.festivalvedy.sk\/wp2\/#website\",\"url\":\"https:\/\/www.festivalvedy.sk\/wp2\/\",\"name\":\"Festival vedy a techniky AMAVET\",\"description\":\"AMAVET - Asoci\u00e1cia pre ml\u00e1de\u017e, vedu a techniku\",\"publisher\":{\"@id\":\"https:\/\/www.festivalvedy.sk\/wp2\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.festivalvedy.sk\/wp2\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"sk-SK\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/www.festivalvedy.sk\/wp2\/#organization\",\"name\":\"Festival vedy a techniky AMAVET\",\"url\":\"https:\/\/www.festivalvedy.sk\/wp2\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"sk-SK\",\"@id\":\"https:\/\/www.festivalvedy.sk\/wp2\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/www.festivalvedy.sk\/wp2\/wp-content\/uploads\/2022\/02\/cropped-fvat-ochranna-zona.png\",\"contentUrl\":\"https:\/\/www.festivalvedy.sk\/wp2\/wp-content\/uploads\/2022\/02\/cropped-fvat-ochranna-zona.png\",\"width\":1190,\"height\":357,\"caption\":\"Festival vedy a techniky AMAVET\"},\"image\":{\"@id\":\"https:\/\/www.festivalvedy.sk\/wp2\/#\/schema\/logo\/image\/\"}},{\"@type\":\"Person\",\"@id\":\"https:\/\/www.festivalvedy.sk\/wp2\/#\/schema\/person\/f6c2e17170a626260fe38db97ed0a1f5\",\"name\":\"Redakcia\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"sk-SK\",\"@id\":\"https:\/\/secure.gravatar.com\/avatar\/2f7c312d560e25e8eb59b6b53cadd5dc16b39f25bac1c7d0a221dafd9c91fae9?s=96&d=mm&r=g\",\"url\":\"https:\/\/secure.gravatar.com\/avatar\/2f7c312d560e25e8eb59b6b53cadd5dc16b39f25bac1c7d0a221dafd9c91fae9?s=96&d=mm&r=g\",\"contentUrl\":\"https:\/\/secure.gravatar.com\/avatar\/2f7c312d560e25e8eb59b6b53cadd5dc16b39f25bac1c7d0a221dafd9c91fae9?s=96&d=mm&r=g\",\"caption\":\"Redakcia\"},\"url\":\"https:\/\/www.festivalvedy.sk\/wp2\/author\/fvatadmin\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Ide\u00e1lna teplota eliminovania inkl\u00faznych teliesok s maxim\u00e1lnym ziskom prote\u00ednu AsLOV2 - Festival vedy a techniky AMAVET","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.festivalvedy.sk\/wp2\/idealna-teplota-eliminovania-inkluznych-teliesok-s-maximalnym-ziskom-proteinu-aslov2\/","og_locale":"sk_SK","og_type":"article","og_title":"Ide\u00e1lna teplota eliminovania inkl\u00faznych teliesok s maxim\u00e1lnym ziskom prote\u00ednu AsLOV2 - Festival vedy a techniky AMAVET","og_description":"Autor\/autori: S\u00e1ra M\u00e1ria Majern\u00edkov\u00e1 \u00davod LOV dom\u00e9ny patria k svetlocitliv\u00fdm prote\u00ednom, ktor\u00e9 vplyvom modr\u00e9ho svetla vytv\u00e1raj\u00fa kovalentn\u00e9 v\u00e4zby s flav\u00edn-mononukleotidom, \u010do sp\u00f4sob\u00ed zmenu konform\u00e1cie prote\u00ednovej \u0161trukt\u00fary. Dan\u00fd proces je reverzibiln\u00fd v tme a preto m\u00e1 vysok\u00e9 potenci\u00e1lne vyu\u017eite v optogenetike. Av\u0161ak produkcia t\u00fdchto prote\u00ednov m\u00f4\u017ee sp\u00f4sobi\u0165 ich hromadenie, pr\u00edpadne nespr\u00e1vne zbalenie v cytoplazme (vznik inkl\u00faznych [&hellip;]","og_url":"https:\/\/www.festivalvedy.sk\/wp2\/idealna-teplota-eliminovania-inkluznych-teliesok-s-maximalnym-ziskom-proteinu-aslov2\/","og_site_name":"Festival vedy a techniky AMAVET","article_published_time":"2019-09-18T10:45:41+00:00","author":"Redakcia","twitter_card":"summary_large_image","twitter_misc":{"Autor":"Redakcia","Predpokladan\u00fd \u010das \u010d\u00edtania":"2 min\u00faty"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/www.festivalvedy.sk\/wp2\/idealna-teplota-eliminovania-inkluznych-teliesok-s-maximalnym-ziskom-proteinu-aslov2\/#article","isPartOf":{"@id":"https:\/\/www.festivalvedy.sk\/wp2\/idealna-teplota-eliminovania-inkluznych-teliesok-s-maximalnym-ziskom-proteinu-aslov2\/"},"author":{"name":"Redakcia","@id":"https:\/\/www.festivalvedy.sk\/wp2\/#\/schema\/person\/f6c2e17170a626260fe38db97ed0a1f5"},"headline":"Ide\u00e1lna teplota eliminovania inkl\u00faznych teliesok s maxim\u00e1lnym ziskom prote\u00ednu AsLOV2","datePublished":"2019-09-18T10:45:41+00:00","mainEntityOfPage":{"@id":"https:\/\/www.festivalvedy.sk\/wp2\/idealna-teplota-eliminovania-inkluznych-teliesok-s-maximalnym-ziskom-proteinu-aslov2\/"},"wordCount":349,"commentCount":0,"publisher":{"@id":"https:\/\/www.festivalvedy.sk\/wp2\/#organization"},"keywords":["2019","Biol\u00f3gia"],"articleSection":["Arch\u00edv 2018","Biol\u00f3gia"],"inLanguage":"sk-SK"},{"@type":"WebPage","@id":"https:\/\/www.festivalvedy.sk\/wp2\/idealna-teplota-eliminovania-inkluznych-teliesok-s-maximalnym-ziskom-proteinu-aslov2\/","url":"https:\/\/www.festivalvedy.sk\/wp2\/idealna-teplota-eliminovania-inkluznych-teliesok-s-maximalnym-ziskom-proteinu-aslov2\/","name":"Ide\u00e1lna teplota eliminovania inkl\u00faznych teliesok s maxim\u00e1lnym ziskom prote\u00ednu AsLOV2 - Festival vedy a techniky AMAVET","isPartOf":{"@id":"https:\/\/www.festivalvedy.sk\/wp2\/#website"},"datePublished":"2019-09-18T10:45:41+00:00","breadcrumb":{"@id":"https:\/\/www.festivalvedy.sk\/wp2\/idealna-teplota-eliminovania-inkluznych-teliesok-s-maximalnym-ziskom-proteinu-aslov2\/#breadcrumb"},"inLanguage":"sk-SK","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.festivalvedy.sk\/wp2\/idealna-teplota-eliminovania-inkluznych-teliesok-s-maximalnym-ziskom-proteinu-aslov2\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/www.festivalvedy.sk\/wp2\/idealna-teplota-eliminovania-inkluznych-teliesok-s-maximalnym-ziskom-proteinu-aslov2\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.festivalvedy.sk\/wp2\/"},{"@type":"ListItem","position":2,"name":"Ide\u00e1lna teplota eliminovania inkl\u00faznych teliesok s maxim\u00e1lnym ziskom prote\u00ednu AsLOV2"}]},{"@type":"WebSite","@id":"https:\/\/www.festivalvedy.sk\/wp2\/#website","url":"https:\/\/www.festivalvedy.sk\/wp2\/","name":"Festival vedy a techniky AMAVET","description":"AMAVET - Asoci\u00e1cia pre ml\u00e1de\u017e, vedu a techniku","publisher":{"@id":"https:\/\/www.festivalvedy.sk\/wp2\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.festivalvedy.sk\/wp2\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"sk-SK"},{"@type":"Organization","@id":"https:\/\/www.festivalvedy.sk\/wp2\/#organization","name":"Festival vedy a techniky AMAVET","url":"https:\/\/www.festivalvedy.sk\/wp2\/","logo":{"@type":"ImageObject","inLanguage":"sk-SK","@id":"https:\/\/www.festivalvedy.sk\/wp2\/#\/schema\/logo\/image\/","url":"https:\/\/www.festivalvedy.sk\/wp2\/wp-content\/uploads\/2022\/02\/cropped-fvat-ochranna-zona.png","contentUrl":"https:\/\/www.festivalvedy.sk\/wp2\/wp-content\/uploads\/2022\/02\/cropped-fvat-ochranna-zona.png","width":1190,"height":357,"caption":"Festival vedy a techniky AMAVET"},"image":{"@id":"https:\/\/www.festivalvedy.sk\/wp2\/#\/schema\/logo\/image\/"}},{"@type":"Person","@id":"https:\/\/www.festivalvedy.sk\/wp2\/#\/schema\/person\/f6c2e17170a626260fe38db97ed0a1f5","name":"Redakcia","image":{"@type":"ImageObject","inLanguage":"sk-SK","@id":"https:\/\/secure.gravatar.com\/avatar\/2f7c312d560e25e8eb59b6b53cadd5dc16b39f25bac1c7d0a221dafd9c91fae9?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/2f7c312d560e25e8eb59b6b53cadd5dc16b39f25bac1c7d0a221dafd9c91fae9?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/2f7c312d560e25e8eb59b6b53cadd5dc16b39f25bac1c7d0a221dafd9c91fae9?s=96&d=mm&r=g","caption":"Redakcia"},"url":"https:\/\/www.festivalvedy.sk\/wp2\/author\/fvatadmin\/"}]}},"_links":{"self":[{"href":"https:\/\/www.festivalvedy.sk\/wp2\/wp-json\/wp\/v2\/posts\/872","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.festivalvedy.sk\/wp2\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.festivalvedy.sk\/wp2\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.festivalvedy.sk\/wp2\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.festivalvedy.sk\/wp2\/wp-json\/wp\/v2\/comments?post=872"}],"version-history":[{"count":0,"href":"https:\/\/www.festivalvedy.sk\/wp2\/wp-json\/wp\/v2\/posts\/872\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.festivalvedy.sk\/wp2\/wp-json\/wp\/v2\/media?parent=872"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.festivalvedy.sk\/wp2\/wp-json\/wp\/v2\/categories?post=872"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.festivalvedy.sk\/wp2\/wp-json\/wp\/v2\/tags?post=872"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}